Coding

Part:BBa_K5250004:Design

Designed by: Ekaterina Tocheva   Group: iGEM24_UZurich   (2024-09-30)


DGC PisoF R196A


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 424
    Illegal PstI site found at 493
    Illegal PstI site found at 711
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 424
    Illegal PstI site found at 493
    Illegal PstI site found at 711
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 144
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 424
    Illegal PstI site found at 493
    Illegal PstI site found at 711
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 424
    Illegal PstI site found at 493
    Illegal PstI site found at 711
    Illegal NgoMIV site found at 732
    Illegal NgoMIV site found at 855
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part has been codon optimized for Pseudomonas species IsoF. At the end of our sequence we added a His-tag for protein purification.

Source

Gene sequence was obtained from a Pseudomonas species IsoF gene bank our host lab provided us with.

References

1. De, N., Pirruccello, M., Krasteva, P. V., Bae, N., Raghavan, R. V., & Sondermann, H. (2008). Phosphorylation-Independent Regulation of the Diguanylate Cyclase WSPR. PLoS Biology, 6(3), e67. https://doi.org/10.1371/journal.pbio.0060067

2. Nie, H., Xiao, Y., He, J., Liu, H., Nie, L., Chen, W., & Huang, Q. (2019). Phenotypic–genotypic analysis of GGDEF/EAL/HD‐GYP domain‐encoding genes in Pseudomonas putida. Environmental Microbiology Reports, 12(1), 38–48. https://doi.org/10.1111/1758-2229.12808